IR injury induces both renal KC and MIP-2 mRNA and protein. Mice underwent renal ischemia followed by 5 to 24 hours of reperfusion or sham surgery. (A,C) KC and MIP-2 mRNA was assessed by RT-PCR and levels expressed relative to 18S ribosomal RNA. (B,D) KC and MIP-2 protein was assessed by ELISA, and levels were expressed as picogram per milliliter (samples were loaded equally for total protein). WT mice undergoing IR injury had significantly elevated KC and MIP-2 mRNA and protein compared with sham-operated WT mice (*P < .001). Low-TF WT mice treated with hirudin and PAR-1^−/− mice had significantly reduced KC and MIP-2 mRNA and protein compared with WT mice (**P < .001). Data are expressed as mean ± SEM.