Defective association of the TCR chains underlies the loss of surface TCR-CD3 expression in RCD cell line P1. SDS-PAGE analysis of immunoprecipitates of RCD cell line P1 and T-cell clone N10 after 35S metabolic labeling. Antisera used were normal rabbit serum as negative control (N), anti–TCR-α (α), anti–TCR-β (β), anti-CD3γ (γ), anti-CD3δ (δ), anti-CD3ϵ (ϵ), and anti-ζ (ζ). (A) Immunoprecipitates obtained from NP40 lysates of RCD cell line P1 were analyzed under reducing (left) and nonreducing (right) conditions. (B) Immunoprecipitates obtained from digitonin lysates of RCD cell line P1 (left) and T-cell clone N10 (right) were analyzed under nonreducing conditions. Positions of individual chains and molecular mass markers (kilodaltons) are indicated. (C) Sequences of the TCR-α and TCR-β chain of cell line P1 (TCRAV14S1, TCRBV11c).
