Correlation of AQP9 expression with As2O3 sensitivity. (A) The quantity of AQP9 mRNA, as determined by Q-PCR, was inversely related to cell survival after treatment in 2 μM As2O3 for 48 hours. ML-1 was an outlier. (B) Western-blot analysis of AQP9 expression. NB4 expressed the highest amount of AQP9 and was used as the standard for comparison in panel C. (C) AQP9 protein expression, as determined by Western-blot analysis using NB4 as the reference, showed an inverse relationship to cell survival. ML-1 was also an outlier.