Effect of LTB4 treatment on surface expression of CCR7. (A) FACS analysis of CCR7 expression on the surface and after permeabilization in iDCs after 30 minutes of LTB4 (1 nM) treatment. Gray line indicates isotype control; broken line, iDCs; and black line, LTB4-treated iDCs. (B) CCL19-Fc fusion protein staining of untreated and LTB4-treated iDCs, both before and after permeabilization, analyzed by confocal microscopy (×100).