HPLC-ED assay of endogenous catecholamines in Tregs and Teffs. Chromatograms of a standard sample containing 300 fmol catecholamines and of each of their metabolites (A) and of representative samples containing endogenous catecholamines and metabolites extracted from 1 × 106 human Tregs (B) and 1 × 106 Teffs (C). Results in Tregs and in Teffs are from 1 representative experiment of 6 separate experiments. For quantitative data, see Table 3.