Inhibition of cellular proliferation of mutant FLT3–expressing cell lines by NVP-AST487. (A) Structure of PKC412 and NVP-AST487. (B) Three-day treatment of FLT3-ITD-Ba/F3 cells, in the presence and absence of IL-3, with NVP-AST487. (C) Three-day treatment of FLT3-D835Y-Ba/F3 cells, in the presence and absence of IL-3, with NVP-AST487. (D) Colony assay formation of human bone marrow progenitor cells in the presence of increasing concentrations of NVP-AST487. Experiments in panels B and C were performed in duplicate; error bars represent SEM.