Inhibition of cellular proliferation of PKC412-sensitive and -resistant mutant FLT3-expressing cells by NVP-AST487. (A) Three-day treatment of FLT3-ITD-Ba/F3 cells with NVP-AST487 or PKC412. (B) Three-day treatment of PKC412-resistant FLT3-ITD-Ba/F3 cells with NVP-AST487 or PKC412 (n = 1 for NVP-AST487 treatment; n = 2 for PKC412 treatment). (C) Two-day treatment of FLT3-ITD-Ba/F3 cells and PKC412-resistant, mutant FLT3-expressing Ba/F3 cells with PKC412 (n = 2). (D) Two-day treatment of FLT3-ITD-Ba/F3 cells and PKC412-resistant, mutant FLT3-expressing Ba/F3 cells with NVP-AST487 (n = 2). Experiments were performed in duplicate; error bars represent SEM.