American Society of Hematology

Figure 2

$Figure 2. Hematopoietic stem cells display p53-dependent differences in proliferation in old mice. (A) Whole bone marrow was labeled with BrdU for 16 hours before numbers of BrdU+, lin−/lo, Sca-1+, or c-kit+ HSCs were assessed by flow cytometry. BrdU incorporation was reduced in HSCs obtained from old p53+/+ and p53+/m mice but not in HSCs from old p53+/− mice. Results shown represent the mean ± SE of 2 experiments using a total of 5 (young) or 7 (old) mice. (B) The fraction of proliferating HSCs in the bone marrow of old mice is dependent on p53 status. The number of HSCs for each p53 genotype in Figure 2B was combined with the fraction of proliferating HSCs for each p53 genotype to determine the total number of proliferating HSCs in the bone marrow for each p53 genotype.$

Hematopoietic stem cells display p53-dependent differences in proliferation in old mice. (A) Whole bone marrow was labeled with BrdU for 16 hours before numbers of BrdU⁺, lin^−/lo, Sca-1⁺, or c-kit⁺ HSCs were assessed by flow cytometry. BrdU incorporation was reduced in HSCs obtained from old p53^+/+ and p53^+/m mice but not in HSCs from old p53^+/− mice. Results shown represent the mean ± SE of 2 experiments using a total of 5 (young) or 7 (old) mice. (B) The fraction of proliferating HSCs in the bone marrow of old mice is dependent on p53 status. The number of HSCs for each p53 genotype in Figure 2B was combined with the fraction of proliferating HSCs for each p53 genotype to determine the total number of proliferating HSCs in the bone marrow for each p53 genotype.

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