Specificity of anti-LMO2 monoclonal antibody and correlation with LMO2 mRNA expression. (A) Immunoblot analysis using the anti-LMO2 monoclonal antibody shows a specific band corresponding to LMO2 protein expression in HeLa cells stably transfected with LMO2 but not in native or mock-transfected HeLa cells. (B) LMO2 mRNA and protein expression were analyzed by real-time RT-PCR in GC-like (SU-DHL-4, OCI-LY7) and in non–GC-like (OCI-LY3, OCI-LY10) DLBCL cell lines and in Raji, Ramos, and HeLa cell lines. Immunoblot analysis shows that LMO2 protein expression corresponds to LMO2 mRNA expression in the cell lines analyzed. Error bars indicate average of 2 triplicate RT-PCR experiments.