Engraftment but lack of MPD in Nf1^−/−, beta c^−/− recipients. (A) Bone marrow from 3 representative irradiated mice given transplants with Nf1^−/−, beta c^−/− (top panel) and Nf1^+/−, beta c^−/− (bottom panel) cells was mostly comprised of cells expressing donor CD45.2, with only a few cells staining positive for CD45.1. Recipients that do not receive donor cells do not stain for the donor-cell marker (data not shown). (B) Flow cytometric analysis of 2 representative mice. Bone marrow and peripheral blood stained for the cell-surface markers Gr-1 and Mac-1, markers commonly associated with mature granulocytes. Similar staining profiles were seen in all recipients. Ranges of double-positive Gr-1/Mac-1 cells in both cohorts, bone marrow 52% to 68% and peripheral blood 12% to 20%. (C) Peripheral-blood analysis monitored over 46 weeks Nf1^+/−, beta c^−/−and Nf1^−/−, beta c^−/− secondary recipients. Animals that display symptoms of MPD would show drastic increases in total WBC counts outside of the range indicated by the bar. Left graph illustrates peripheral-blood analysis monitored over 56 weeks Nf1^+/−, beta c^−/− and Nf1^−/−, beta c^−/− secondary recipients. Upper lines are lymphocyte percentages and lower lines are neutrophil percentages. Animals that display symptoms of MPD would show drastic increases in neutrophil percentages and reductions in lymphocytes, with values outside of the normal ranges indicated by the dotted (lymphocyte) and solid (neutrophil) bars. Four data points included for Nf1^+/+, beta c^+/+ primary recipients to illustrate normal values.