Hematology parameters of mice reconstituted with CXCR4^+/+ and CXCR4^–/– FL cells. Lethally irradiated C57BL/6-Ly5.1 mice received transplants of 5 × 10⁶ cells per mouse. CXCR4^+/+ and CXCR4^–/– mice displayed similar levels of peripheral chimerisms. (A) Blood samples were analyzed from 2 to 16 weeks after transplantation for the hematology parameters using an MS9 Counter. Each point represents the mean ± SD of absolute numbers scored from mice that received CXCR4^+/+ transplants (n = 6; ▴, CXCR4^+/+) and mice that received CXCR4^–/– transplants (n = 5; ▦, CXCR4^–/–). (B) Representative flow cytometry analysis of peripheral blood leukocytes in CXCR4^+/+ and CXCR4^–/– mice 2 and 8 weeks after transplantation. Cells were dually labeled with either mAb Gr-1 to detect granulocytic cells or mAb B220 to detect B lymphocytes and anti-CD45.2 to detect donor-derived cells. The same animals were followed at 2 and 8 weeks after transplantation. The percentages are calculated based on total viable blood cells.