Effects of the Slc35d3, Rab27a, and agouti (A) genes on pigmentation. Control C57BL/6J (A) and C3H/HeSnJ (B) mice display typical nonagouti (a) and agouti (A) pigmentation, respectively. The ash-Roswell (C) and ash-Jax (D) mutants, which arose on the C3H/HeSnJ inbred line, are hypopigmented because of the homozygous Rab27a (ashen) mutation. The Slc35d3 mutation does not affect coat or eye color when transferred in homozygous form to the C57BL/6J (E) or C3H/HeSnJ (F) backgrounds.