GSK-3 specifically enhances IL-12p35 mRNA expression and inhibits IL-10 mRNA expression. Real-time PCR quantification of mRNA in MoDCs stimulated with BHK-CD40L cell line as in Figure 3 with or without the GSK-3 inhibitor SB415286. Specificity of the mRNA signal was controlled by adding BHK-CD40L cells to the unstimulated MoDCs (0 h) and by analyzing the influence of mock-transfected BHK cells (no CD40L) (n = 1, not shown) on cytokine mRNA expression levels. Shown are the means ± SD; n = 3, normalized to the 24-hour mRNA levels without inhibitor. *P < .05, **P < .01 (comparison with and without SB415286).