Figure 1.
Schematic diagram for generation of functionally active anti-Aspergillus T lymphocytes. After stimulation of 1 × 108 PBMCs with the cellular extract EC SAB of A fumigatus, IFN-γ-producing cells were isolated using the IFN-γ secretion assay. Anti-Aspergillus T cells either were further expanded or were used to generate functionally active T cell clones by limiting dilution. Characterization of the functionally active T cells included flow cytometry, including intracellular, antigen-triggered cytokine staining, expansion on restimulation, hyphal damage, and assessment of alloreactivity.
