MS4a4B protein is detected by FACS analysis. Cells were isolated, stained for surface markers where indicated, fixed and permeabilized, and stained with anti-MS4a4B (solid line) or rabbit Ig (light line with shading) as control. (A) Spleen cells stained for the indicated lineage markers. (B) Staining results for T-cell clones (5cc7, T32, AE7, CTLL-2, HT-2) and hybridomas (58α and α28) are shown. (C) Staining results for Mac1⁺ cells, freshly isolated from spleen, analyzed immediately (fresh), or expanded in vitro with MCSF (expanded) for 7 days before analysis. Results shown are representative of 3 separate experiments.