Clonal multipotentiality of SSEA-4⁺ cells. Multilineage differentiation of a representative mesenchymal stem cell clone obtained from SSEA4⁺ cells isolated from human BM aspirates. Fat (A), bone (B), and cartilage (C) differentiation were observed by Oil Red O, Alizarin Red S, and Alcian Blue staining (top), respectively, as well as by relevant gene expression analyses (bottom). Original magnification, 10×/0.3 NA Ph1.