Emodin and DHA reduce the level of phosphorylated Rb and induce G₁ cell-cycle arrest in HTLV-I–transformed cells. (A) Cell-cycle analysis of propidium iodide–stained HTLV-I–transformed C8166 cells treated with As₂O₃/IFN-α in the absence or the presence of emodin and DHA. (B) Western blot analysis of phosphorylated Rb in C8166 and 1185 cells treated with As₂O₃/IFN-α in the absence or the presence of emodin and DHA or emodin and DHA alone. Actin was used as loading control.