Plasma containing HNA-3a antibodies prime the fMLP-activated respiratory burst of HNA-3a+ PMNs. (A) The maximal rate of superoxide anion production as a function of treatment group includes 10% plasma (final concentration = percentage, vol/vol) from 4 donors (numbered 1-4) with antibodies to HNA-3a, all of whom were implicated in TRALI reactions compared with 10% fresh plasma (FP; isolated from 4 healthy donors: 2 males and 2 multiparous females). Immune complexes generated by heat-treating human serum for 30 minutes at 56°C were used as a positive control and could be inhibited (by 97.4% ± 8.3%) by preincubation with a mixture of (Fab′)2 fragments from murine monoclonal antibodies against human CD16, CD32, and CD64. The data are expressed as the mean ± the standard error of the mean of 8 separate experiments using disparate donors. (*P < .05 compared with FP.) (B) The maximal rate of superoxide anion concentration as a function of concentrations (final percentage, vol/vol) of plasma with antibodies to HNA-3a. HNA-3a plasma (3%-10%) significantly primed the fMLP-activated respiratory burst compared with FP- and buffer-treated controls; however, 1% did not (*P < .05 compared with FP- or buffer-treated controls). PAF is used as a positive control and this figure is representative of the data from all 4 plasma samples that contained IgG antibodies to HNA-3a (Abs).
