The BOB.1/OBF.1 CpG island is methylated in cHL cell lines. Analysis of the BOB.1/OBF.1 CpG island by methylation-specific PCR (MSP). Bisulfite-converted DNA samples were used for amplification of the BOB.1/OBF.1 CpG island. The sequence of the 5′-untranslated region of BOB.1/OBF.1 was found by alignment of the longest mRNA sequence Z49194 to genomic DNA. The primers, specific for methylated (MF and MR) and unmethylated (UF and UR) CpGs were positioned in the CpG-rich area inside exon 1. Thin vertical lines represent the positions of CpG dinucleotides. The PCR amplification products specific for methylated (M) and unmethylated (U) DNA (138 bp and 216 bp, respectively) were separated on 6% polyacrylamide gels. MSP data shown are representative for 3 independent experiments.