Expression of FGF receptors in stromal cells. Expression of FGFRs in MS-5 and S-17 cells was detected by RT-PCR. Total RNA extracted from MS-5 cells and S-17 cells was subjected to RT-PCR using specific primer pairs. RNA quality was evaluated in all samples by parallel RT-PCR for GAPDH. Absence of contaminating genomic DNA was ensured by RNA-PCR. PCR products were separated on a 2% agarose gel prestained with 1 μg/mL ethidium bromide and visualized under UV light. Representative results from at least 2 independent experiments are shown.