Effects of FGF family members on stromal cell secretion of SDF-1. (A) MS-5 cells and S-17 cells (80%-90% confluent in 96-well plate) were incubated in culture medium (αMEM containing 10% FBS, 200 μL/well) alone, or with FGF2, FGF4, FGF7, FGF9, FGF16, or FGF19 (50 ng/mL) for 72 hours; cell-free culture supernatants were collected and SDF-1 content was measured by a specific ELISA. All experiments were performed in triplicate and means (± SD) were calculated. The percent SDF-1 secretion was calculated as follows: (SDF-1 secretion in FGF group/SDF-1 secretion in control group with no additive) × 100 (%). The results reflect the means (± SD) of 3 to 4 independent experiments. The asterisk denotes statistical significance (**P < .01). (B) MS-5 cells and S-17 cells were detached and washed twice with PBS. Cells were suspended in culture medium (αMEM containing 10% FBS), plated (2000 cells/well in 0.2 mL culture medium) in triplicate wells (96-well plates) with the addition of FGFs (50 ng/mL), and incubated for 64 hours. DNA synthesis was measured by 3 H thymidine deoxyribose uptake during the last 16 hours of culture. The percent 3 H incorporation was calculated as follows: (3 H incorporation in FGF group/3 H incorporation in control group with no additive) × 100 (%). The results reflect the means (± SD) of 3 to 4 independent experiments.
