Montage of low magnification confocal images of spleen sections of LTα^−/− mice. (A) LTα^−/− mice. (B) Organization of the spleens of LTα^−/− mice 10 days after transfer of CD4⁺CD3⁻ cells. CD3 is shown in red and IgM in green. Yellow shows colocalization of B and T cells. Inset boxes show single staining for VCAM-1 (in white) in the T zones as outlined by white figures in main pictures. Scale bar represents 100 μm. Results representative of at least 2 separate experiments.