Evidence that CD4⁺CD3⁻ cells are associated with VCAM-1⁺ stromal cells. Confocal images of spleen sections showing (A) wild-type (WT) and (B) LTα^−/− mice. Dotted yellow area of panel A identifies B follicles in WT mice. To show association of VCAM-1⁺ cells with CD4⁺CD3⁻ cells, confocal images from T-cell–deficient mice were analyzed at low magnification (10×) (C), high magnification (63×) of B-cell area (D), and high magnification of T-cell area (63×) from panel C (E). Scale bar represents 50 μm for low magnification, 20 μm for high magnification.