Evaluation for p110δ protein and activity in thymocytes. (A) Representative immunoblots of class 1a and 1b p110 subunits expressed in thymocytes harvested from WT control and p110γδ^-/- mice. Western blot of β-actin illustrates equal loading of proteins. (B) Detection of Akt/PKB in Western blots of total lysates from p110γ^-/- thymocytes treated with vehicle control or the p110δ-specific inhibitor IC87114 (10 μM) before TCR cross-linking. (C) Ca²⁺ flux in CD4⁺-gated thymocytes in WT control, p110γ^-/-, p110δ^-/-, and p110γδ^-/- mice in response to TCR cross-linking. Ca²⁺ flux in CD4⁺CD8⁺-sorted thymocytes from WT control and p110γδ^-/- animals is shown for comparison (inset). Data are representative of 3 to 4 separate experiments.