Analysis of splenic NK cells in ZGKR mice. (A) The number of NK cells per spleen isolated from individual mice of the indicated genotype is presented. The numbers were calculated based on the number of splenocytes and the percentage of CD3^–MHC class II^–NK1.1⁺ cells present in the lymphocyte gate. Dots indicate individual mice; horizontal bars, mean values. No statistically significant differences were observed with analysis by unpaired Student t test (data not shown). (B) Expression of the indicated NK cell markers was analyzed on gated CD3^–MHC class II^–NK1.1⁺ splenocytes isolated from indicated mice. Represented mice also express comparable levels of Ly49 C/I receptors (data not shown). Data are representative of 3 independent experiments (CTR indicates negative control mAb).