Role of LAT and NTAL in LAK effector functions. (A-B) LAK NK cells were purified from control (CTR) and mutant mice and used as effectors against indicated tumor cells. Redirected killing of Daudi cells by purified LAK cells isolated from control (CTR, ○ and ▵) and LAT/NTAL^KO double KO (• and ▴) has been performed in the presence of isotype control mAbs (isot. ctr.) or anti-Ly49D mAbs (α-Ly49D). Data shown are representative of at least 2 independent experiments. (C) Purified LAK cells isolated from indicated mice were incubated for 24 hours on plates previously coated with anti-Ly49D mAb or isotype control mAb. Cell-culture supernatants were then harvested, and IFN-γ contents were determined by ELISA. Basal values detected in the presence of isotype control mAb were subtracted from values obtained via Ly49D stimulation. Data shown are representative of 5 to 8 independent experiments. Horizontal bars indicate mean values.