Sp1 activation of PDGF-D transcription is Ets-1 dependent. (A) SMCs were cotransfected with 2 μg CMV-Sp1 and backbone vector CMV-gutless together with 10 μg p1168PDGF-Dluc, with or without 2 μg dominant-negative Ets-1 (DN-Ets-1) and its backbone pKCR3. (B) CMV-Sp1 and/or pcDNA-Ets-1 (1 or 2 μg, as indicated) were transiently cotransfected into SMCs alone or simultaneously together with 10 μg p1168PDGF-Dluc. The data are representative of at least 2 independent experiments. Error bars represent SEM performed in duplicate or triplicate. *P < .05 relative to control using Student t test.