Rearranged immunoglobulin variable region heavy chain and light chain sequence analysis of 20 single sorted cells from CLL subject 151. Sequence alignment for 8 subgroups (A-H) of the VH3-23 (A), Vk2-A19/A3 (B), and VL8a (C) immunoglobulin genes sequenced from 20 single CLL cells from subject 151. Complete paired sequencing data from the VH3-23, Vk2-A19/A3, and VL8a immunoglobulin genes were available from only 20 single CLL cells. This accounts for the different numbers presented in this figure compared with Table 2. Dashes signify identical bases, and dots represent the absence of bases compared with the germ line genes for VH3-23, Vk2-A19, and VL8a. Replacement mutations are shown in uppercase, and silent mutations are shown in lowercase.