Intracellular cytokine analyses in pDCs and CD11c⁺ mDCs in response to TLRL plus CD40L. pDCs and CD11c⁺ mDCs purified by cell sorter to more than 99% purity were cultured with TLRLs plus CD40L. (A) After 8 hours, intracellular cytokine production by activated pDCs and CD11c⁺ mDCs was analyzed by flow cytometry. (B) After 8, 10, and 12 hours of culture, cytokine production by activated pDCs was analyzed by flow cytometry. Percentages of the cytokine-producing DCs are indicated in each dot blot profile. Data are from 1 of 3 independent experiments.