BDCA4⁺pDCs isolated by magnetic beads contain CD11c⁺mDCs that contribute to IL-12 production. (A) pDCs isolated by cell sorter or by BDCA4-conjugated magnetic beads were analyzed by staining with anti-CD123, anti-BDCA2, anti-CD11c, and anti-HLA-DR mAbs. Data shown are from 1 of 4 independent donors. (B) Using the cells isolated by BDCA4-conjugated magnetic beads, intracellular cytokine production and surface CD11c expression were analyzed by flow cytometry after 8 hours of stimulation with CpG plus CD40L. Numbers in each dot blot profile indicate the percentages of the indicated fractionated cells. Data are from 1 of 3 independent experiments.