Functional analysis of HIV tetramer–positive cells and their Vβ populations ex vivo. (A) IFN-γ secretion of Vβ2 and Vβ14⁺ CD8⁺ T cells in AC-46 ex vivo. Percentages indicate the frequency of IFN-γ⁺ cells per CD8⁺ T cells (left) or the frequency of Vβ⁺ cells within the IFN-γ⁺ population (right). (B) Kinetics of B8-FL8 tetramer–positive cells and IFN-γ⁺ cells after stimulation with B8-FL8 peptide in AC-46 during STI. Bars indicate relative frequency (%) of IFN-γ⁺ cells within the B8-FL8 tetramer⁺ population. (C) Costaining of B8-FL8 tetramer and intracellular IFN-γ secretion in Vβ2 and Vβ14⁺ CD8⁺ T-cell populations in AC-46 ex vivo after stimulation with B8-FL8 peptide or PMA/ionomycin. (D) Summary of the IFN-γ secretion of B8-FL8 tetramer⁺ Vβ2⁺ and Vβ14⁺ CD8⁺ T cells after stimulation with B8-FL8 peptide in 3 independent experiments. (E) Costaining of B8-FL8 tetramer and intracellular IFN-γ secretion in 4 Vβ⁺ populations in AC-15 ex vivo after stimulation with B8-FL8 peptide. (F) Bar graph indicates the frequency of IFN-γ⁺ cells per Vβ population.