Permanent graft survival of GalT-mediated BMT mice. BMT mice received a heterotopic heart transplantation from WT (αGal⁺) mice 4 weeks after BMT (n = 5 for GalT and REV mice). Syngeneic hearts (WT to WT, GalT^–/– to GalT^–/–; n = 5 each) were not rejected during a period of 100 days (data not shown). (A) Graft survival assessed by Kaplan-Meier method. (B) The rejected grafts in REV BMT mice show severe focal inflammatory cell infiltration and myocardial necrosis in hematoxylin and eosin (HE)–stained sections (original magnification × 100). Grafts from GalT BMT mice that were killed at 100 days after heart transplantation showed no signs of rejection.