BM stromal cells show higher levels of transduction and chimerism compared with hematopoietic cells. (A) BM cells and PBCs were collected from killed GalT-transduced BMT mice. αGal expression was assessed by flow cytometry and genomic integration of GalT was assessed by quantitative PCR (n = 5). (B) Chimerism levels in BM stromal cells (SC) were higher than in hematopoietic cells (HC) (n = 5). BM cells were cultured in retronectin-coated plates for 24 hours and αGal expression and genomic integration of GalT were compared in harvested adhesive cells (SC) and nonadhesive cells (HC) (n = 5). (C) Transduced cells were harvested individually as adhesive cells and nonadhesive cells 5 days after transduction. αGal expression and genomic integration of GalT were high in stromal cells (n = 10 each). Data are shown as mean ± SE.