Phenotypes of normal and myeloma-treated BMDCs. (A) Expression of DC-related molecules on normal DCs (medium control) and BMDCs cultured in the presence of TCCM, 5TGM1 cells on Transwell insets, or irradiated 5TGM1 cells in a direct coculture. (B) Expression of CD86 and MHC class II on normal mature BMDCs (medium control) and on cells in cultures with the addition of TCCM from 5TGM1, MOPC-315, or MPC-11 myeloma cells. Cells from d10 cultures were analyzed. Values above histograms represent mean fluorescence intensity. Representative results of 4 experiments are shown. ^*P < .05; ^**P < 0.01 (compared with normal controls).