Impaired neutrophil but not macrophage influx in peritonitis. (A) FACS analysis of day 1 peritoneal cells. Peritoneal cells were stained with FITC–anti–Mac-1 and APC–anti–Gr-1. Numbers indicate the percentages of cells in the gated regions. Also shown are cytospin images of peritoneal cells from Mcl-1^−/− and control mice. (B) FACS analysis and cytospin images of day 4 peritoneal cells. (C) Numbers of total peritoneal cells, neutrophils, and macrophages at day 1 and day 4 after thioglycollate injection. The numbers of Mac-1⁺Gr-1⁺ and Mac-1⁺Gr-1⁻ cells were calculated by multiplying the percents of cells with the total numbers (n = 6). Data shown are mean + standard deviation. (D) Expression of Mcl-1 in purified peritoneal neutrophils from Mcl-1^−/− and control mice as determined by Western blot. Erk2 serves as a loading control. Data are representative of 2 experiments.