Messenger RNA expression as measured by real-time PCR. (A) Real-time RT-PCR confirmation of genes of interest: HES6, GPR160, RUNX2, and FLT3. (B) Real-time RT-PCR reveals differential expression of SIGLEC3 (CD33) and SIGLEC6 (CD33-like1) when comparing CD4⁺CD56⁺HN and c-AML. Expression values are presented as relative to RPS11 expression.