Disruption of Survivin ΔEx3 results in a diminished angiogenic response in vivo. (A-C) DIVAA was performed as previously described. Angioreactors (A) were prefilled with extracellular matrix with or without the angiogenic factors heparin and FGF-2 (negative and positive controls) or containing angiogenic factors and supplemented with IgG control or Survivin ΔEx3 antibody, then implanted into the dorsal flanks of athymic nude mice. (B) After 11 days, the angioreactors were removed and photographed under a dissecting microscope to establish blood vessel invasion. (C) Quantification of angiogenic responses was performed after recovery of cell pellets from the angioreactors following dispase digestion and staining with FITC-lectin. Results represent the mean fluorescence ± SE from 4 replicates.