TLR-specific activation of BEAS-2B cells by Gram-positive bacteria. (A) Dose-dependent induction of IL-6 secretion by heat-inactivated bacteria (L monocytogenes, B subtilis, and S aureus) in BEAS 2B cells. Error bars show ± SEM. (B) Inhibitory effects of anti-TLR4, anti–MD-2, anti-TLR2, and anti-CD14 mAbs in the activation of cells by 2 × 108/mL Gram-positive bacteria (L monocytogenes, B subtilis, and S aureus; 3 different experiments). Duplicates were performed for all conditions tested. Horizontal lines represent the mean.