Endogenous degradation of CD33 and SOCS3. (A) The adherent monolayer from PBMCs was treated for 2 hours with LPS (100 ng/mL), incubated with α-CD33 (IC7/1) for 1 hour, and cross-linked with GAM for 2 hours. (Ai) Lysates were immunoprecipitated with α-CD33 and immunoblotted with α-CD33 (IC71/3D6). (Aii) Lysates were immunoprecipitated with α-SOCS3 and immunoblotted with α-SOCS3 (M20). (Aiii) WCL was immunoblotted with α-STAT5B as a loading control. (B) Signal intensities of CD33 and SOCS3 expression levels were normalized to signal intensity of STAT5B and are illustrated as graphs.