Type I IFN production and viral replication in MHV-infected splenic cDCs and pDCs. (A) Flow cytometric analysis of splenic cDCs (CD11c⁺PDCA-1⁻) and (B) splenic pDCs CD11c^low B220⁺ PDCA-1⁻ before FACS sorting. Gates for sorting are indicated. (C) Primary FACS-purified murine splenic cDCs or pDCs were infected with MHV at a multiplicity of infection (moi) of 1. IFN-α secretion to culture supernatants was determined by ELISA at the indicated time points. (D) Virus titers in culture supernatants were determined by plaque assay. (C-D) Data represent mean values ± SD pooled from 2 experiments. Statistical analysis was performed using Student t test (*P < .05; **P < .01).