Effect of WNT pathway inhibition on GSF3β phosphorylation status. SP53 and Jeko-1 cells were treated with different concentrations of natural Wnt inhibitor: sFRP1 (A) or sFRP4 (B). Treatments with either of these 2 sFRP proteins led to a significant decrease in p-GSK3β. The bottom graph on each figure represents densitometry measurement of the p-GSK3β expression levels. Error bars indicate SD.