PKCβII is overexpressed by CLL cells. Quantitative analysis of PKCβII protein and mRNA expression. (A) Analysis of PKCβII protein in CLL, HCL, and CD19-purified normal B cells. (B) Analysis of PKCβII protein in CLL cells from patients with the indicated chromosomal abnormalities. 11q23 and 17p13 deletions were combined because these abnormalities are typically associated with severe disease.²⁹  13q14 deletion and trisomy 12 chromosomal abnormalities were grouped with normal karyotypes because these abnormalities are not associated with severe disease.²⁹  (C) Analysis of PKCβII protein in CLL cells from patients with different disease stages at the time of sampling. (D) Analysis of PKCβII protein in relation to white blood cell count. (A-D) PKCβII protein levels are expressed as a percentage of total cellular protein. (E) Analysis of PKCβII mRNA by quantitative RT-PCR in CLL cells and HCs. Results are expressed relative to β-actin mRNA in the same cell. Tests for statistical significance were performed with the Mann-Whitney U test.