KAHRP is trafficked normally to the erythrocyte membrane and assembled into knob structures. (A) Immunofluorescence assay to determine the localization of KAHRP in parental CS2 (top) and CS2ΔSBP1-infected erythrocytes (bottom). Cells were reacted with anti-KAHRP antibodies and identical localization patterns for both parasite lines were observed. (B) Scanning electron microscopy revealed typical knob structures in CS2ΔSBP1 parasite-infected erythrocytes compared with parental CS2. The panel shows a representative trophozoite-infected erythrocyte of each cell line. The bar represents 2 μm. (C) Ultrastructural analysis of CS2- and CS2ΔSBP1-infected cells of knob structures (arrow).