In vivo hematopoietic reconstitution ability of clone-3 SSEA-1⁺ cells. Representative flow-cytometry plots of bone marrow engraftment analysis from 1 of the recipient mice. The assessment of donor-derived cells was determined by the presence of MHC-I–expressing cells. (A-B) NOD/SCID-β2^null and NOD/SCID BMMNCs were used as negative and positive controls, respectively. BMMNCs from each recipient animal were stained with an appropriate isotype control (C) and MHC-I antibodies (D) to determine the percentage of donor-derived cells. Multicolor FACS analysis shows that most donor-derived cells (MHC-I⁺; gated area of D) were of hematopoietic origin, composed mainly of CD45⁺ cells (E). Most donor-derived CD45⁺ cells coexpressed the myeloid antigen Gr-1 (F) and, conversely, were also positive for lineage-committed antigens (G). Some cells with KLS (c-Kit^low/+/Lineage⁻/Sca-1⁺) phenotype were also found (G-H).