Strong eosinophil activation enhances TF mRNA. (A) Full-length TF mRNA levels are increased in response to eosinophil stimulation. Sequential activation of isolated eosinophils (act eos; 5 × 105) with GM-CSF (50 ng/mL; 20 minutes), followed by PAF (10 μM; 25 minutes), increases the mRNA expression for TF (top panel, conventional RT-PCR; bottom panel, real-time PCR). Comparisons with the mRNA levels in LPS-stimulated monocytes (act mono), resting eosinophils (eos), platelets (P), and PBLs. The expression levels were calculated as the ratio of TF mRNA to aldolase mRNA. Means ± SEM, n = 3. (B) GM-CSF/PAF causes ECP release. Degranulation of specific granules by GM-CSF (50 ng/mL; 20 minutes), and by GM-CSF plus PAF (10 μM; 25 minutes), as verified by ECP secretion. Means ± SEM, n = 4. *P < .05 (versus control).
