Figure 5
Differential effects of anti-TF antibodies on eosinophil accumulation and transmigration under flow conditions. Endothelial cells treated for 24 hours with 20 ng/mL IL-4 (20 ng/mL) were assembled into the flow chamber, and freshly isolated eosinophils (5 × 105/mL) were perfused over the monolayer at 2 dyn/cm2 for 4 minutes. In some experiments, the endothelial cells and eosinophils were pretreated for 15 minutes with VIC7 or VD8 (10 μg/mL). Representative images of eosinophils bound to IL-4–treated endothelial cells in (A) control, (B) VIC7, and (C) VD8 are shown at ×200 magnification. Transmigrated cells are indicated with white arrows. Data are representative of at least 3 independent experiments.
