Priming to one alloantigen can result in the elimination of third-party BM indicating nonspecificity in the priming response. (A) B6 mice were primed against BALB/c or B10.BR (BR) by the intraperitoneal administration of 20 × 10⁶ splenocytes, lethally irradiated on day −1 (8.0 Gy), and infused with BALB/c or BR BM (20 × 10⁶) on day 0. Survival is shown. n = 8/group. (B) Serum was pooled from BALB/c- or B10.BR-primed B6 mice (n = 5) and incubated with BALB/c or B10.BR BM. Histograms illustrate binding of serum antibody to BM of priming strain (top panels) and third-party strain (bottom panels). Percentage positive binding is given. Negative control is shown by thin dashed line.