CLL mAb with stereotypic IGHV1-69, IGHD3-16, and IGHJ3 rearrangements immunoprecipitate MYHIIA. Antibodies from CLL068 (2 different recombinant mAb preparations) or human IgG were used to immunoprecipitate HEp-2 cell extracts. Immunoprecipitate (IP) and supernatant (Sup) samples were electrophoresed in 6% SDS-PAGE as in Figure 1. After blotting to nitrocellulose, the membrane was probed with rabbit antihuman anti-MYHIIA as shown.