Glut-1 expression on IL-7–stimulated RTEs reflects increased glucose uptake. CD4⁺ RTEs were cultured for 120 hours in the presence of rIL-7 doses ranging from 0.01 to 100 ng/mL (A). Alternatively, the time during which cells were exposed to rIL-7 (10 ng/mL) during the 120-hour incubation was varied, ranging from 0 hours to 120 hours (B). Glucose uptake was assayed by incubating cells (5 × 10⁵) with 2-deoxy-D[1-³H]glucose (0.1 mM) for 45 minutes at 37°C. Uptake is expressed as mean counts per minute (CPM) for triplicate samples; error bars indicate SD. Data are representative of results obtained in 4 independent experiments.