Effect of hrAnx-A1 on T-cell activation. (A) Murine naive lymph node T cells were stimulated with 5.0, 2.5, and 1.25 μg/mL anti-CD3/CD28 in the absence or presence of different concentrations of hrAnx-A1 for 24 hours and then pulsed with [³H]-thymidine to measure cell proliferation. (B) IL-2 production from primary murine naive lymph node T cells stimulated with anti-CD3/CD28 (1.25 μg/mL) in the absence or presence of different concentrations of hrAnx-A1 for 24 hours. (C, D) Murine naive lymph node T cells were stimulated with the indicated concentration of anti-CD3/CD28 in the absence (top panels) or presence (bottom panel) of hrAnx-A1 (600 nM) for 12 hours and then analyzed for CD25 and CD69 expression by FACS. Graphs on the left summarize the results obtained with 150, 300, and 600 nM Anx-A1. In all the experiments, values are mean ± the standard error (SE) of 4 to 5 mice. *P < .05; **P < .01.